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SplitsTree is a widely used application for computing unrooted phylogenetic networks from molecular sequence data. Given an alignment of sequences, a distance matrix or a set of trees, the program will compute a phylogenetic tree or network using methods like split decomposition, neighbor-net, etc.
The aim of SplitsTree4 is to provide a framework for evolutionary analysis using both trees and networks. The program takes as input a set of taxa represented by characters (that is, aligned sequences), distances, quartets, trees or splits and produces as output trees or networks using a number of different methods.